So, I found a strange data from my RNA-seq analysis. I found that p53 is upregulated in cancer cell line compare to normal cell line. I found this is really strange because usually p53 is down regulated in cancer cell line. Is it possible I messed up with my DESeq function call? Comparing to another gene, I think other gene expression result is normal. Is it biologically explainable? I'm thinking probably the upregulation is caused by a drug. Maybe you can share yoour experience. Thank you.