Hi I will be very grateful if you are so kind answer me about 2 question from population differentiation method. 1- What can be interpreted from a negative Theta value (-1) when measuring pairwise Theta value (Fst) between 2 populations? 2- I have 2 animal breeds with several sub-populations. I analyse my data with Theta value in 2 breeds and their sub-populations. Why Theta value is different between sub-populations and breeds?? for subpopulations must analysis with Fsc value????