i got a question to ask you guys.recently our lab focused on a type of secreted protein to predict and its identification,then i searched the related articles then i found the paper titled
Genomewide identification of proteins secreted by the Hrp type III protein secretion system of Pseudomonas syringae pv. tomato DC3000
in this paper, the author used software Artemis to predict the secreted effector. i never used this type of software before and so do the other guys in our lab,after times and times of trial i still didn't know how to use it.
could you please help me out with it, step by step.