I would like to blast my sequences using NCBI standalone blast. I downloaded the lates version of blast(2.2.31+).
After downloading I extract the files and installed according to the instruction at the website. It was installed under
this folder contain the uninstaller , bin and doc. Then I downloaded the databases "refseq_rna.00" and create a folder of "db" within the installed blast.
Then I configured my PCy creating the the enviornment variabe "path" and giving the location of
Afterwords I opened the cmd fom my system and to check wheather the installation is ok I used the following commands.
Microsoft Windows [Version 6.1.7601]
Copyright (c) 2009 Microsoft Corporation. All rights reserved.
C:\Users\Ashar Hussain>cd /
C:\>cd Program Files/NCBI/blast-2.2.31+
Volume in drive C has no label.
Volume Serial Number is 40E5-5989
Directory of C:\Program Files\NCBI\blast-2.2.31+
06/19/2015 10:06 AM <DIR> .
06/19/2015 10:06 AM <DIR> ..
06/18/2015 12:51 PM <DIR> bin
06/22/2015 12:27 PM <DIR> db
06/18/2015 12:51 PM <DIR> doc
06/19/2015 09:59 AM 62,465 Uninstall-ncbi-blast-2.2.31+.exe
1 File(s) 62,465 bytes
5 Dir(s) 228,627,599,360 bytes free
C:\Program Files\NCBI\blast-2.2.31+>cd bin
C:\Program Files\NCBI\blast-2.2.31+\bin>blastn -version
Package: blast 2.2.31, build Jun 2 2015 10:18:08
Until this point this is working fine but when I want to check the databases I downloaded consol is giving the messages like this
C:\Program Files\NCBI\blast-2.2.31+\bin>blastdbcmd -db refseq_rna.00 -info
BLAST Database error: No alias or index file found for nucleotide database [refs
eq_rna.00] in search path [C:\Program Files\NCBI\blast-2.2.31+\bin;;]
I tried to resolve the error by downloading the databases again but it didno't resolved.
Does anybody has idea? any suggetions?
thanks in advance