I would like to blast my sequences using NCBI standalone blast. I downloaded the lates version of blast(2.2.31+).
After downloading I extract the files and installed according to the instruction at the website. It was installed under
This folder contains the uninstaller, bin and doc. Then I downloaded the databases
refseq_rna.00 and create a folder
db within the installed blast.
Then I configured my PCy creating the the environment variable "path" and giving the location of
Afterwords I opened the cmd from my system and to check whether the installation is ok I used the following commands.
Microsoft Windows [Version 6.1.7601] Copyright (c) 2009 Microsoft Corporation. All rights reserved. C:\Users\Ashar Hussain>cd / C:\>cd Program Files/NCBI/blast-2.2.31+ C:\Program Files\NCBI\blast-2.2.31+>dir Volume in drive C has no label. Volume Serial Number is 40E5-5989 Directory of C:\Program Files\NCBI\blast-2.2.31+ 06/19/2015 10:06 AM <DIR> . 06/19/2015 10:06 AM <DIR> .. 06/18/2015 12:51 PM <DIR> bin 06/22/2015 12:27 PM <DIR> db 06/18/2015 12:51 PM <DIR> doc 06/19/2015 09:59 AM 62,465 Uninstall-ncbi-blast-2.2.31+.exe 1 File(s) 62,465 bytes 5 Dir(s) 228,627,599,360 bytes free C:\Program Files\NCBI\blast-2.2.31+>cd bin C:\Program Files\NCBI\blast-2.2.31+\bin>blastn -version blastn: 2.2.31+ Package: blast 2.2.31, build Jun 2 2015 10:18:08 C:\Program Files\NCBI\blast-2.2.31+\bin>
Until this point this is working fine but when I want to check the databases I downloaded console is giving the messages like this
C:\Program Files\NCBI\blast-2.2.31+\bin>blastdbcmd -db refseq_rna.00 -info BLAST Database error: No alias or index file found for nucleotide database [refs eq_rna.00] in search path [C:\Program Files\NCBI\blast-2.2.31+\bin;;]
I tried to resolve the error by downloading the databases again but it didn't resolve.
Does anybody has idea? Any suggestions?
Thanks in advance