I'm using Tophat to align RNA-Seq reads to a genome. I want to know how many reads aligned, but I didn't know about the -g option when I ran Tophat, so the normal commands like "samtools -c accepted_hits.bam" isn't giving me the number of unique reads in the file, it's giving me the total number of alignments. Is there any way I can get this information without re-running Tophat?
Question: Get # of unique mapped reads from Tophat output
3.8 years ago by
colin.kern • 220
colin.kern • 220 wrote:
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