Question: Poly(A) Tail From Rna-Seq?
2
gravatar for Leszek
2.4 years ago by
Leszek2.9k
Barcelona, Spain
Leszek2.9k wrote:

Is it common to find poly(A) tail in RNA-Seq reads? And can this information be used to get better splice variants assignment?

ADD COMMENTlink written 2.4 years ago by Leszek2.9k
1
gravatar for Larry_Parnell
2.4 years ago by
Larry_Parnell15k
Boston, MA USA
Larry_Parnell15k wrote:

I am not certain of good responses to your two questions, but I have read that length of the poly(A) tail can vary according to conditions within the cell. Thus, noting the length of poly(A), if you have accurate measures from RNA-Seq, between control and various conditions may be an important characteristic of your gene expression data to retain.

ADD COMMENTlink written 2.4 years ago by Larry_Parnell15k
1
gravatar for Wen.Huang
2.4 years ago by
Wen.Huang870
Wen.Huang870 wrote:

I have seen poly A reads in my own limited data.

The following paper has specifically looked at poly A reads as a way to get alternative poly A sites

http://www.nature.com/nature/journal/v464/n7289/full/nature08872.html

ADD COMMENTlink written 2.4 years ago by Wen.Huang870
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