Hello everyone. I am new to bioinformatics, although i have been around these forums for a while.
As for my question, I am working with protein sequences from completed genomes of a bacteria, but I have noticed that something weird (or interesting) happens to the downloaded proteomes and genomes that I dowload from the NCBI site.
After the downloads are completed, for [Ref_Seq] sequences, two things happen: either an error message appear at the end of the sequence as “Resource temporarily unavailable (4).”, or every file I download has a different amount of lines, gene amounts and data size.
Is this normal?. Maybe is not statistically significant in the analysis? Is there any way to improve this?
Thank you. And apologies for my grammar