I am performing RNAseq analysis using TopHat and Cufflinks programs. I have a question for why a gene I am interested is being called Not Significant by Cuffdiff.
Here the output for my gene of interest Rh4:
gene sample_1 sample_2 status value_1 value_2 log2(fold_change) test_stat p_value q_value signific Rh4 WT MUT OK 1158.87 0.856892 -10.4013 -4.12026 0.1178 0.404333 no
I am not familiar with the statistics Cuffdiff uses to call my gene of interest Not Significant. Can someone explain it to me?
Thank you very much.