Question: How To Combine Two Types Of Affymetrix Genechip Results?
0
gravatar for Gangcai
7.5 years ago by
Gangcai230
Berlin Or Shanghai
Gangcai230 wrote:

Dear All, I have two sets of Affymetrix GeneChip data, one from GeneChip® HT MG-430 PM Array Plate and another from GeneChip® Mouse Genome 430 2.0 Array. According to official description, they use the same kind of technology, however they have different probset ids and different probe-sequences. My question is how can I combine those two datasets for comparison?

Thanks in advance.

gene affymetrix probeset • 2.3k views
ADD COMMENTlink modified 7.1 years ago by boczniak767640 • written 7.5 years ago by Gangcai230
1

Gangcai, you can join your data as shigeta wrote and try metaanalytic approach, i.e. something to transform data from different experiments to the same scale. Take a look at metaArray, GeneMeta, RankProd, metahdep packages. [?]Somewhere in these tutorials is example of joint analysis of several Affymetrix experiments.[?] HTH

ADD REPLYlink written 7.5 years ago by boczniak767640

This can be done by taking your log2 difference results from the 2 separate arrays and taking the identical probe set names and comparing them. The two have almost the same probe set contents. Any differences, if any, are not likely to be of biological consequences.

As with other microarray data, scaling data from different experimental runs can be done (esp in this case as they are essentially the same array), but often don't get the best results because of variance between samples, prep, hybridization conditions, etc.

ADD REPLYlink written 7.5 years ago by Shigeta460

Hi shigeta, thanks for your reply. I have checked the probeset names, and only 64 probes have exact same ids between those two GeneChips.

ADD REPLYlink written 7.5 years ago by Gangcai230

there is a resource on affymetrix.com called a comparison spreadsheet. they should be available for these 2 arrays - they are less exact unfortunately as the probes are not identical.

ADD REPLYlink written 7.5 years ago by Shigeta460
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