Hi all,
One of worse issues during transcriptome assembly is when two transcripts are fused together by their ends, in this case we will find two separate ORFs within the same contig with different BLAST hits (one blast hit for positive strand and another for negative strand). My question is how to identify these contigs within the assembly?, after that how to deal with such contigs, they have to keep or discard? your informative response would be highly appreciated in advance.
Thanks friend, I try it. Just one issue, the authors set
-max_target_seqs
100 in their blastx followed by chimer detection in the paper. As by setting this number, blastx take longer time to finish. this number (100) is important for chimer detection in your view?. I usually run blastx with-max_target_seqs
1 or 5. Please share me your opinion?Thanks
I think it is pretty important to have large number of target matches. Because the way it detects chimera depends on the target alignment support (although 100 might be too many, and can be done with lesser target matches, I would definitely not use 1 target matches at all).