FastUniq "error in open left fastq file for read!"
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Entering edit mode
7.6 years ago
ae75 • 0

I'm getting the following error with FastUniq (running for PE sequences, testing with just one pair):

Error in open left fastq file *FULL TEXT OF FIRST READ IN FIRST FILE* for read!


There are a few other complaints of this problem scattered on the web but none have even a suggestion for solving it. Does anyone have any ideas for how to resolve this issue? Or, alternatively, want to suggest another tool for filtering duplicates in fastq format?

Assembly RNA-Seq • 2.8k views
0
Entering edit mode
7.6 years ago

You can use Dedupe from the BBMap package instead. Usage:

dedupe.sh in1=r1.fq in2=r2.fq out=nodupes.fq


For paired input, the output will be interleaved, but you can deinterleave it with reformat.sh if you want.