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• What size should I expect from a SFF file?
  I am building a FastQ visual editor tool ( https://www.biostars.org/p/98851/ ) and I want to inte...
• How To Convert Fastq/Sff To Fasta With Quality Cut Off
  Hi all~ I want to extract sequence from fastq/sff with quality cut off for avoiding poor quality...
• Add Simulated 454 Reads To The Fastq File Generated By Sff_Extract For Use With Mira
  I created a FASTQ and Traceinfo XML file from a set of .sff files using sff_extract. Now I want t...
• Efficiently process (view, analize, clip ends, convert, demultiplex, dereplicate) SFF/FastQ files
  Hi. I am a biologist in need of a good graphic/visual/fast FastQ editor. Starting from a Biostars...
• Sfffile Program Of Roche 454
  hi there!! I need to remove mids and mid sort my sff files while retaining its sff format. I i...
• 454Newblermetrics.Txt Format
  Assembly with newbler gives a summary file, 454NewblerMetrics.txt that according to documentation...
• Sff File With Multiple Genome
  Hi Does anyone have a sff file or know where I can find sff file with short reads sequenced from ...
• Simulate Sff File
  Hi, I am giving a workshop of genome assembly and I would like to have the students try genome as...
• How to remove primers from a Roche 454 sff file
  Good morning, I am implementing a pipeline for Roche 454 Junior and I have few questions, I hope...
• Generate percent of bases with quality > x from BAM file
  I am interesting in finding the percent of bases with quality > x from a BAM file.  I want to ...
• Please Suggest Good Read To Understand Sff To Fasta Conversion
  hello all, I have earlier worked with EST sequences, tools for assembly, gene finding ,etc. I am...
• Retrieve MiSeq Data still containing index primers etc
  Hi there We have a pipeline that we have developed that currently works on both 454 and Ion Torr...
• Whole genome assembly of Ion torrent single-end data
  Hello all, My goal is to do whole genome assembly of a fungal taxonomy, I have fastq file reads ...
• Biopython Not Indexing (Or Parsing) Full .Sff File, Why?
  Please examine the following code (I've changed some things for privacy). >>> from Bio ...
• Newbler Statistics
  Hello I've done an assembly with newbler 2.5 under -cdna option. This assembly use reads from 3 ...
• Generating Genome Base Qualities From A Bam Alignment?
  Has anyone ever tried to generate base qualities for a next-gen genome assembly using a .bam alig...