Is it possible to generate a reference from exome sequenced/aligned(BWA MEM 0.7.10) to map the RNAseq data from the same individual?The idea is to reduce the mapping bias in RNA seq mapping to the common hg19 reference.
I would appreciate some advice/suggestions on tools at different steps.
Thanks in advance
Yes, it is possible. This question has already been discussed several times on this forum. Please use the search feature to find them. At the same time I will keep the question open to get some fresh insights into this topic here.