Chip-Chip peaks calling
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6.5 years ago

Hi 

I am new to ChIP-ChIP analysis. Can you please help me to understand which files from this experement are control samples and which ones are tratment?  (the organism is d.melanogaster) 

 

Link to the data http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1283/samples/?s_sortby=col_39&s_sortorder=ascending

 

 

chip-chip drosophila • 1.4k views
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Have you read the experiment description?

"Genomewide mapping of D. melanogaster Lame duck protein binding at 6-8hrs after egg laying. Two different rabbit antibodies were used to precipitate the Lmd protein isoform in biological replicates. Additionally a rabbit preimmune-serum was used as a control. The enriched DNA was hybridized to high density Affymetrix GeneChip Drosophila Tiling 1.0R array."

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Entering edit mode
6.5 years ago
Ian 5.8k

The preimmune sample are the controls (this is sera taken from the animals before challenge with the antigen).

The description from http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1283/ says:

"Two different rabbit antibodies were used to precipitate the Lmd protein isoform in biological replicates. Additionally a rabbit preimmune-serum was used as a control."

I hope this helps.

 

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