Best 16S Region ?
2
0
Entering edit mode
12.6 years ago
Dph ▴ 10

Does someone have an idea of the best 16S gene region to amplify with NGS in order to assess microbial (bacterial) diversity in environmental samples ? cheers

metagenomics • 2.6k views
ADD COMMENT
0
Entering edit mode

What do you mean best? Do you refere to selecting from among the universal primers? http://en.wikipedia.org/wiki/16S_ribosomal_RNA#Universal_Primers

ADD REPLY
3
Entering edit mode
12.6 years ago
Zhidkov ▴ 600

You can use information from The Ribosomal Database Project (RDP):[?] http://rdp.cme.msu.edu/ [?] or Silva database[?] http://www.arb-silva.de/ [?]

Ilia

ADD COMMENT
1
Entering edit mode
12.6 years ago

Our colleagues use one 16S amplicon across the V3-V5 region.

ADD COMMENT
0
Entering edit mode

and some other use the V6 one...

ADD REPLY

Login before adding your answer.

Traffic: 3637 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6