Tool: Homology Analysis Tool - An analysis tool that can be used for both BLAST and RDP classifier for NGS metagenome data
gravatar for sun1014child
23 months ago by
United States
sun1014child0 wrote:

Homology Analysis tool takes either fasta or fastq files, exported as next generation sequencing (NGS) data by sequencers, to execute either BLAST or RDP classifier for your metagenome analysis. It includes not only 16S rRNA database from NCBI gbbct but also database from DDBJ or GreenGene. This user-friendly application is free to download and facilitates your metagenome analysis. 

This tool provided free of charge courtesy of World Fusion. It was originally created as a companion tool for Metagenome@Kin, an automated metagenomic data analysis tool that measures the composition and diversity of microbial and fungal species from NGS data. Easy to learn & implement, no manual commands required.

For more information visit :!homology-analysis-tool/cjia

Contact details:


Phone(World Wide): +1-619-542-7879 

blast tool rdp 16s next-gen • 1.4k views
ADD COMMENTlink modified 22 months ago • written 23 months ago by sun1014child0

I haven't had a chance to use your BLAST/RDP GUI, but thanks for notifying us of the tool.  

Not to be nitpicky, so excuse me for speaking up, but this is a major pet peeve of mine.  RDP classifier is used to identify ribosomal sequences from 16S primers, so I wouldn't call it a metagenomics tool as you are not probing genomic data, you're looking at the amplification products of a single gene.  I don't know how your companion tool (Metagenome@Kin) works, but for your Homology Analysis Tool which you list here, the 16S portion is not considered metagenomics.  Also, it's 16S, as 'S' in Svenberg units.

ADD REPLYlink modified 23 months ago • written 23 months ago by Josh Herr5.4k

Hi Josh, 


Thank you for your comment. I fixed "s" to "S". Sorry for my ignorance. If you have time to use the Homology Analysis Tool and some feedback, I'll appreciate it. Also, if you are interested in Metagenom@KIN, there is a free version(for two week once you install). Here is the info:!metagenomekin/c1khf

Thank you very much

ADD REPLYlink written 23 months ago by sun1014child0

No problem, didn't mean to be critical, just wanted to be clear and correct some issues with your wording and communication.

I'm giving the tools a spin, thank you for your tool development and for letting us know about them!

ADD REPLYlink written 23 months ago by Josh Herr5.4k

You're very welcome!

ADD REPLYlink written 23 months ago by sun1014child0
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