Hi, can anyone help? i started using trimmomatic 0.33 today so I'm not that familiar with it. i have paired end data and after checking the quality of my reads i tried trimming for the adapters and low quality using trimmomatic. the adapters used for the my sequences are nextera. i followed the example that was used online for trimming trueseq adapters on an ngs and after trimming the adapters were still detected in my sequences. this is the command i used for my run: java -phred trimmomatic-0.33.jar PE -phred 33 TW_R1.fastq.gz TW_R2.fastq.gz TW_R1trimmed.fastq.gz TW_R2trimmed.fastq.gz ILLUMINACLIP:NexteraPE-PE.fa:2:30:10: LEADING: 3 SLIDINGWINDOW:4:15 MINLEN:40.
Question: TRIMMOMATRIC Nextera trimming
5.0 years ago by
meekadi • 10
meekadi • 10 wrote:
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