Obtaining RIN scores with Agilent Bioanalyzer's mRNA program

Question

[Transcript]

Hello everyone,

I'm checking the RNA quality from a microbiome extraction on the Agilent Bioanalyzer. I ran the mRNA program so that I could see the % of rRNA present in the sample compared to total RNA.

However, the Bioanalyzer program for mRNA does not provide a RIN (RNA Integrity Number) score, unlike the Total RNA program. Is there a way to obtain a RIN score for traces from the mRNA program? The electropherogram should be the same; I just need a way to apply the algorithm for calculating RIN.

Anyone know how to get the Bioanalyzer program to do this quality check?

[GouthamAtla]

May be this post suites better on Seq answers.

[Transcript]

I'll give it a shot over there, too. Trying to cast a wide net.

[GouthamAtla]

but cross posting is not encouraged. :)