Question: Obtaining RIN scores with Agilent Bioanalyzer's mRNA program
0
gravatar for Transcript
5.5 years ago by
Transcript0
California, USA
Transcript0 wrote:

Hello everyone,

I'm checking the RNA quality from a microbiome extraction on the Agilent Bioanalyzer.  I ran the mRNA program so that I could see the % of rRNA present in the sample compared to total RNA.

However, the Bioanalyzer program for mRNA does not provide a RIN (RNA Integrity Number) score, unlike the Total RNA program.  Is there a way to obtain a RIN score for traces from the mRNA program?  The electropherogram should be the same; I just need a way to apply the algorithm for calculating RIN.

Anyone know how to get the Bioanalyzer program to do this quality check?

ADD COMMENTlink written 5.5 years ago by Transcript0

May be this post suites better on Seq answers.

ADD REPLYlink written 5.5 years ago by geek_y11k

I'll give it a shot over there, too.  Trying to cast a wide net.

ADD REPLYlink written 5.5 years ago by Transcript0

but cross posting is not encouraged. :)

ADD REPLYlink written 5.5 years ago by geek_y11k
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