global alignment using needleall
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Entering edit mode
8.7 years ago
Quak ▴ 490

I am using EMBOSS implementation of needleman to build a similarity matrix of 50k nucleotide reads against each other;

  1. I don't know how to get the final alignment result from EMBOSS implementation of needleman (it returns the score) and I don't see any parameter to specify the alignment file in the output
  2. What would be the most efficient way of doing this. 1 read against n-1 reads, for all n reads? (it doesn't sound so efficient, specially since the needleall, can handle alignment of many reads against many reads) ...
alignment needleall • 1.7k views
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