I'm trying to follow a procedure described in this paper: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1716261/
Specifically their parameters for BLAST which they say are "match = +5, mismatch = −4, gapopen = 10, gapextension = 10". However when I try to run blastn with these parameters, it doesn't let me use them, saying
BLAST engine error: Error: Gap existence and extension values 10 and 10 are not supported for substitution scores 5 and -4 10 and 6 are supported existence and extension values 8 and 6 are supported existence and extension values 25 and 10 are supported existence and extension values Any values more stringent than 25 and 10 are supported
Why doesn't it allow these parameters? Is it likely that the paper has a typo in the parameters they report, or did older or alternate version of BLAST support these?