Off topic:Differentailly expressed genes of microarray time series data with replicates...
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8.7 years ago
alpha09 ▴ 10

I have micro array data which is RMA normalized. I want to extract differentially expressed genes.

My data is times series and with replicates.below is the format of my data..(0,4,8,12,16,20) is one day expression and (24,28,32,36,40,44) is 2nd day.

Gene_ID  LDT0     LDT4     8        12       16       20       24       28       32       36       40       44
LHY      2243.22  481.2    7.19888  4.78697  10.6717  150.279  1904.97  483.612  8.46222  6.00153  10.8894  135.684
MEA      3.49595  4.04554  3.77595  4.06207  4.03678  4.17023  3.08672  4.18224  3.86883  4.13705  3.54243  4.44046
GBF4     47.3822  72.3035  64.8164  58.9292  52.0097  47.802   54.1476  38.7481  54.5051  83.3657  57.8709  51.4214
CRY2     875.509  839.057  1135.12  1050.2   905.306  1027.61  923.662  836.94   1104.79  1045.74  895.141  1075.92
NFYC9    312.643  337.326  340.071  308.826  252.628  259.479  317.697  295.563  333.879  292.991  319.404  279.003
PIF3     78.576   108.083  68.376   92.5676  32.0555  16.3228  29.381   114.997  124.115  75.6262  61.7131  10.8697
sequence next-gen gene R • 1.0k views
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