Question: integrated Network analysis using WGCNA - queries
0
gravatar for Mamta
4.8 years ago by
Mamta450
United States
Mamta450 wrote:

HI all,

I have a dataset for proteomics, miRNA, mRNA profiling for three disease groups and controls. I want to see the similarities and differences between networks. 

Few suggestions I would need before proceeding-

1. Do i do each dataset separately or out them all together and analyze?

2. Do I run all the phenotypes together?

3. For miRN- do I do the negatively correlated pairs before adding?

You help is much appreciated!

Mamta

ADD COMMENTlink modified 4.8 years ago by Adamc640 • written 4.8 years ago by Mamta450

Also, if anyone is familiar with the wgcna tutorial. I was trying to see the module association with clinical traits (here I have types, age, group, subject rather than any numerical measurement unlike the tutorial) Was wondering If i can still see the module correlation to these traits. In my current analysis, it just gives me NAs . I am not sure why.

Thanks,
Mamta

ADD REPLYlink modified 6 months ago by RamRS27k • written 4.8 years ago by Mamta450
1
gravatar for Adamc
4.8 years ago by
Adamc640
United States
Adamc640 wrote:

1) Each dataset (analysis type) can be run separately and you can look for module conservation or determine some other correlation/connection between the modules for the individual datasets later. It wouldn't make sense to run them together because the data/units aren't on the same scale and the identifiers are likely different for each.

2) If the phenotypes have anything to do with each other (derived from the same tissue, or from the same patients, etc) then it makes sense to run them together. If there's no logical relationship between the phenotypes (if comparing phenotypes wouldn't be something you're interested in) then they should be run separately.

3) By this do you mean filter the miRNA for ones that are significantly regulated in a direction that is opposite of their targets? It might make sense. I'd probably try it both ways.

ADD COMMENTlink written 4.8 years ago by Adamc640

Thanks Adamc. That makes sense and I am going to try that.

Mamta

ADD REPLYlink modified 6 months ago by RamRS27k • written 4.8 years ago by Mamta450
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