Question: Transgene insertion using ChIP-seq data
0
gravatar for sergio.espeso-gil
3.7 years ago by
Spain
sergio.espeso-gil0 wrote:

Hi community,

 

I have ChIP-seq data from different histone marks and I think I can get the insertion sites of a trangene by clipping out the sequences from the extremes of the transgene. I have been trying to do it by mapping the transgene promoter using bowtie and trying to get the sequences, but it didn't work out.  I did 50bp sequencing single strand.

 

Any ideas?  

 

Thanks, 

 

Sergio

 

chip-seq next-gen gene • 939 views
ADD COMMENTlink modified 3.7 years ago by Devon Ryan89k • written 3.7 years ago by sergio.espeso-gil0
1
gravatar for Devon Ryan
3.7 years ago by
Devon Ryan89k
Freiburg, Germany
Devon Ryan89k wrote:

You'll need to do microassembly (e.g., with mapsembler) using the transgene sequence. The biggest problem with this will be if your ChIP signal is very sharp or not near a transgene edge then you won't get enough coverage to find the insertion site(s). Just google around for microassemblers. You will also have problems if you have too many insertion sites (e.g., you're sequencing a highly diverse population), but there's no way around these issues given the data you apparently have.

ADD COMMENTlink written 3.7 years ago by Devon Ryan89k

Thanks! That is a good start with. I will try. 

ADD REPLYlink written 3.7 years ago by sergio.espeso-gil0
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1239 users visited in the last hour