Question: Exome-seq: many reads with mate pair mapping to different chromosome
gravatar for murphy.charlesj
4.1 years ago by
United States
murphy.charlesj90 wrote:

I have exome-sequencing from mouse tumors as well as constitutional DNA from the tails (Illumina, 50bp paired end). Tumor sequencing data looks good, but the data from the tails does not. One thing in particular is the percentage of reads with a mate pair that maps to a different chromosome. In tumors this percentage is around 0.2%, while in the tail samples it is around 15%--that is, 15% of the reads that have a paired read that mapped to a different chromosome. I'am going to investigate this further, but I wanted to know if anyone has anyone else encounter a similar problem? I' am thinking to just simply remove those 15% of the reads from the tail samples.

Thank you.

sequencing next-gen • 1.2k views
ADD COMMENTlink modified 4.1 years ago by Brian Bushnell16k • written 4.1 years ago by murphy.charlesj90

I do not know the answer, but I think you should made this as a 'Question' instead of 'Forum'. Mostly people that could answer will skip this topic thinking that is not a question.

ADD REPLYlink modified 4.1 years ago • written 4.1 years ago by tiago2112871.1k

You're right, thanks!

ADD REPLYlink written 4.1 years ago by murphy.charlesj90
gravatar for Brian Bushnell
4.1 years ago by
Walnut Creek, USA
Brian Bushnell16k wrote:

At a guess, I'd say you have the samples switched.  How is the read quality?

ADD COMMENTlink written 4.1 years ago by Brian Bushnell16k
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