Entering edit mode
8.7 years ago
zizigolu
★
4.3k
friends,
I downloaded TopHat Linux x86_64 binary and unzipped with tar -xzvf tophat-2.1.0.Linux_x86_64.tar.gz
I have already installed bowtie2 and samtools
I did like so, toptah is in the same folder with bowtie2 and samtools and my reference genome (orf_coding.fasta
), indexed genome and read (SRR2046309_trimmed.fastq
) are in the another folder
I don't know what I should do hereafter to run tophat
[izadi@lbox161 ~]$ bash
[izadi@lbox161 ~]$ cd /usr/data/nfs6/izadi/gij
[izadi@lbox161 gij]$ pwd
/usr/data/nfs6/izadi/gij
[izadi@lbox161 gij]$ export TOP=/usr/people/home/izadi/fereshteh/tophat-2.1.0.Linux_x86_64
[izadi@lbox161 gij]$ echo $TOP
/usr/people/home/izadi/fereshteh/tophat-2.1.0.Linux_x86_64
[izadi@lbox161 gij]$ $TOP/tophat coding orf_coding.fasta SRR2046309_trimmed.fastq
[izadi@lbox161 gij]$
After running the above syntax no happen I saw...
Help me please
Thanks Goutham
I did like so, toptah is the same folder with bowtie2 and samtools and my refertence genome (
orf_coding.fasta
), indexed genome and read (are in the another folderI don know what I should do hereafter to run tophat
after runing the above syntax no happen I saw...
help me please
Have you looked at the TopHat manual?
You will need to minimally create bowtie2 indexes (using bowtie2-build) for the genome you are trying to use. You can't use fasta genome file directly with TopHat.
I indexed already...
Then use the "Basename" of the index (unless that name is
coding orf_coding.fasta
in this case). BTW: Is there a space in that name? Not a good idea. Or ifcoding
is the basename of the index then you don't need to include the name of the genome fasta.From TopHat manual:
thank you I did
$TOP/tophat coding SRR2046309_trimmed.fastq
but again no happenWhat happens when you just do
That should print a bunch of help commands to the screen.
If it does not then you may need to add execute permissions to tophat program by doing
sorry I don know how I can use tophat for mapping
Can you retry to see if the tophat help gets printed to screen?
Are you sure the tophat executable is in
/usr/people/home/izadi/fereshteh/tophat-2.1.0.Linux_x86_64/
? It may be further down inbin
directory. This will require you to runthank you
even I downloaded the test file but even on them no happen I can see after running tophat
Why don't we forget about the TOP variable and try this straight command. This should print some help text to the screen.
If this does not work then the pre-compiled binary is not compatible with your fedora OS. You will need to download the source and compile the program yourself.
sorry I think I unzipped wrongly then I cant find the tophat2 inside the unzipped file