Question: Suggestion for de novo transcriptome assembly of a plant with high heterozygosity
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4.9 years ago by
seta1.3k wrote:

Hi all,

I've got Illumina sequencing reads (100 bp, PE) from a non-model dioecious plant (two genotypes, one is sensitive to treatment and another is tolerant) with high heterozygosity. What's your suggestion for de novo transcriptome assembly and RNA-seq analysis given the high heterozygosity of sample? Is it better to pool all reads or using separate reads from two genotypes for de novo assembly? Thanks for your comments

sequencing rna-seq assembly • 1.4k views
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