Hi all,
I sequenced the complete mitochondrial genome of a species and i annotated it using MITOS. Then i used ORF Finder to verify MITOS Annotation. My question is how to verify if ORF Finder prediction is right, because if i look at the sequence i can find start-stop codons before and after ORF Finder prediction.. I thought maybe to performe an alignment with other mt genomes from close related species and make comparisons, but is there another way? maybe some accurate method that im not aware of?
thanks for the help!