Question: how to chose one 16s rRNA from each genome
0
gravatar for akhilvbioinfo
5.1 years ago by
akhilvbioinfo150
India, chennai
akhilvbioinfo150 wrote:

 I predicted all 16s rRNAs using rnammer . for each genome there is more than two 16s rRNAs ..so how to choose one 16s RNA for each genome form this? in what base? the selected 16s RNAs will used to MSA and phylogeny analysis
 

rna-seq gene • 1.4k views
ADD COMMENTlink modified 5.1 years ago by 5heikki9.0k • written 5.1 years ago by akhilvbioinfo150
1
gravatar for 5heikki
5.1 years ago by
5heikki9.0k
Finland
5heikki9.0k wrote:

You could cluster them and apply the same criteria for choosing the representative sequence in all cases, e.g. most abundant or centroid. Or you could just pick the longest, random, the one with the highest score against the hmm, etc..

ADD COMMENTlink modified 5.1 years ago • written 5.1 years ago by 5heikki9.0k
0
gravatar for Abdullah
5.1 years ago by
Abdullah100
Germany
Abdullah100 wrote:

one suggestion would be to take them all and make a consensus sequence out of them, and then use this for further analysis.

ADD COMMENTlink written 5.1 years ago by Abdullah100
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1016 users visited in the last hour