Crossposted from Bioconductor:
Sorry for this naive question, but I am new to principal component analyses and how to display them. I have two different gene sets, coding and non-coding genes, and count data from two different conditions (four replicates of both). I want to compare how the samples cluster in the two gene sets, i.e. do they have the same expression signatures. I made PCAplots of each gene set separately in DESeq2, but I wonder if it makes sense at all to plot them in the same PCA-plot?
Thanks, Jon
That was my feeling to, thanks for the answer!
The two plots would most likely not be comparable because PC1/PC2 would be different between the two PCAs. If you want to see how the samples cluster between two conditions and be able to say something about the similarity/difference then you should analyze the two datasets jointly.