How can I find out if a gene's function has been altered in an organism? What's the proper way to do it?
Details of what I've done: I blasted the prortein and used uniprot to look at its function and compared the functions to the first few highly similar organisms' functions? Is this the right way? This way I'm finding more than 3 functions that are novel in the organism of interest.
Somebody suggested me to to look at the mutated site from blast result and check for its annotation. Now I'm only a beginner in bioinfo and I'm not sure how to look for the annotation associated with that specific site.
Any help would be greatly appreciated.