I am implementing a pipeline for Roche 454 Junior and I have few questions, I hope that someone could help me :)
I do not know the structure of reads that are in the SFF file, I think that they are composed by MID- Primer 1- Sequence - Primer 2- MID, it is wrong? Primer1 and Primer2 are different or same?
Could you suggest a good tool to remove primers?
Thank you in advance for your attention!