Localize TFBS, Search annotation
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8.6 years ago
jaqy ▴ 20

hello,
i have a set of validated TFBS, and i would like determine each TFBS in these promoter sequence how can i localize TFBS in these promotor ? (if i have a consensus sequence how can i localize the experimentally validated cis-regulatory element present in a specific promoter ?
thank you in advance

genome alignment sequence Assembly gene • 1.6k views
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You have to look for the binding motif in your sequences. There are plenty of tools that can do that, e.g. MEME suits. Anyway, look into BIOSTARS there are many similar questions.

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thank you for your reply,

but, i want to localize the experimentally validated cis-regulatory element present in a specific promoter, i think if i use MEME for exemple, it finds predicted motifs, may be a false positive.

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You can look DBs that have these informations, e,g, TRANSFAC

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