Off topic:Mechanism Of Transgene Integration (From Expression Vector To The Host Genome)

Question

[Saeid Kadkhodaei]

Hi All,

May I know if you have any idea/comment/reference answering the following question:

How does a transgene (in vector) integrate to the host genome? (e.g. in glass bead method, neither biolistic nor agrobacterium).

I already cut some parts (NdeI-PciI) of the vector (pUC18) and wondering if it affects the integration procedure. Is there any recombination-like procedure same as gateway?

Thanks in advance...