Question: (Closed) Mechanism Of Transgene Integration (From Expression Vector To The Host Genome)
gravatar for Saeid Kadkhodaei
8.8 years ago by
Saeid Kadkhodaei90 wrote:

Hi All,

May I know if you have any idea/comment/reference answering the following question:

How does a transgene (in vector) integrate to the host genome? (e.g. in glass bead method, neither biolistic nor agrobacterium).

I already cut some parts (NdeI-PciI) of the vector (pUC18) and wondering if it affects the integration procedure. Is there any recombination-like procedure same as gateway?

Thanks in advance...

genetics • 1.9k views
ADD COMMENTlink modified 8.8 years ago by Neilfws49k • written 8.8 years ago by Saeid Kadkhodaei90


ADD REPLYlink written 8.8 years ago by Pierre Lindenbaum131k

Not a bioinformatics question sorry; ask in a molecular biology forum.

ADD REPLYlink written 8.8 years ago by Neilfws49k
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