Entering edit mode
8.6 years ago
muna2175
▴
10
I am writing a project proposal for the very first time and I need all the help I can .My topic is 'In-silico studies of meta-signature genes in lung cancer'.
Here I need to know, after the process of identifying meta signature genes 'in-silico', how do I make a data base of these meta-signatures.what is the procedure for submitting it to the data base?
It would be very gratifying to receive an answer as soon as possible.
Define 'meta signature' gene in contrast to a 'signature' gene. First things first, how you implement a data-base is much further down the road.
Microarray analysis generates hundereds of Signature genes that are differentially expressed in cancer and thus making it difficult to distinguish the genes that play a critical role in the neoplastic phenotype from those that represent epiphenomena or are spuriously differentially expressed.
Meta-analysis compares and assesses the intersection of those hundered of signature genes,and reveals genes that are relevant to a particular type of cancer.Those intersected genes are the meta-signature genes for a particular cancer.(Rhodes et al)
Michael Dondrup has been kind to start the Q & A link. I was wondering when would Michael go down the road of implementing a database for the meta-signature genes.
IF I had funding for this undertaking ;) but first I would google for existing databases. I mean it might fit in NAR db issue, and there is one: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2808880/
In the end it is maybe not exactly what you want, you can download a bunch of csv files there which not only contain the data for your gene but also all others in the meta analysis. I would think it could be further improved by making it more graphical and interactive, but potentially the large number of different platforms supported makes this hard.
For your personal usage it might be useful to import data for the genes of interest into a local SQL database to make comparing profiles easier.
Thank you very much Michael!