Entering edit mode
8.6 years ago
haiying.kong
▴
360
There are 2 ways of generating .dict file for human genome.
java -jar picard.jar CreateSequenceDictionary REFERENCE=reference.fa OUTPUT=reference.dict
samtools faidx ref.fasta
I found that the 2 files generated in the 2 different command have different file size.
I need .dict file for GATK and Picard. Which one is correct one?
Thank you very much.
The files created by both the tools are different. I guess u need both .fai and .dict to use .fa as a reference
I am not able to understand how to create a dictionary by using the latest version of Picard 2.8.1. It is different from the previous version.
Should I use i for input instead of REFERENCE and what will be option for output?
Old Picard 1.8.1 Command:
New Command options: