Question: Read Orientations in IGV view of Bisulfite Sequencing
gravatar for DVA
4.4 years ago by
United States
DVA530 wrote:

I'm kinda new to the bisulfite mode feature in IGV - I wonder if anyone here knows what determines the gray (5'-3') and green (3'-5') orientations when load a sam file to IGV. It does not seems to be explained on their official website. Is the orientation still fully determined by the flag number, like in normal sequencing cases please? 

Thank you!

igv bisulfite sequencing • 1.4k views
ADD COMMENTlink modified 4.3 years ago by vassialk190 • written 4.4 years ago by DVA530
gravatar for vassialk
4.3 years ago by
vassialk190 wrote:

Try other viewers like Ugene, Galaxy, CLC, GeneousPro

ADD COMMENTlink written 4.3 years ago by vassialk190
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