Is it normal that my RNA degradation plot shows more degradation at the 3'-end?

Question

[Juan Cordero]

Dear comm,

I have made a RNA degradation plot with the function plotAffyRNAdeg from affy package (Bioconductor) and it seems RNA is more degraded at the 3'-end compared with the 5'. What does it mean? Should I trust my results? Should I correct anything?

BTW, all p-values from summaryRNAdeg function are below 0.05.

Thanks in advance

[Chirag Nepal]

Show the figures so others can give their opinion.

But in general, RNA quality towards 3'ends are of lower quality with respect to 5'-ends.

[Juan Cordero]

I get a plot with an opposite trend to this one: lower values in the x-axis get higher values in the y-axis, and vice versa.