Aligning ChIP-seq reads to repeats: IgG vs input controls
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8.6 years ago
jotan ★ 1.3k

I would like to check if I have ChIP-seq reads that map to repeats in the genome (e.g. LINEs, SINEs, telomeres, centromeres etc.) I use the Kharchenko lab repeat aligner to do this.

I intuitively feel as if an Input sequencing control is the best control since this should give an idea of the copy number of repeats within a single genome. However, I have noticed that some people use an IgG control instead.

Are there any thoughts on which control is better for alignments to repeats specifically?

ChIP-Seq • 2.2k views
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