Question: Phred Score/Base quality score in Pysam
0
gravatar for Lylthera
4.7 years ago by
Lylthera0
Germany
Lylthera0 wrote:

Hi,

I'm examining the phred quality scores for bases of certain snps, so I wonder how to get this information - I'm using pysam and there's the method that provides quality scores (for example query_alignment_qualities or query_qualities. 
In the doc it says, these are  "aligned query sequence quality values (None if not present).", (or "read sequence base qualities", respectively). Does anybody know it these are phred scores, or if not, how would base quality else be computed in this implementation? Thanks in advance. 

ADD COMMENTlink modified 4.7 years ago by glihm620 • written 4.7 years ago by Lylthera0
0
gravatar for glihm
4.7 years ago by
glihm620
France
glihm620 wrote:

Hi Lylthera,

Yes, these are Phred scores. In the page 40 of this book you will find the confirmation. The Phred score is printed only for the aligned part.

ADD COMMENTlink modified 6 months ago by RamRS27k • written 4.7 years ago by glihm620
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