Question: The first amplicon librariesis is needed to analyse the 16S data?
0
gravatar for m.koohi.m
3.0 years ago by
m.koohi.m70
United States
m.koohi.m70 wrote:

Hi,

For analyzing 16S data with Mothur or Qiime is it necessary that I have the the first amplicon libraries sequences or not? The data is produced by MiSeq illumina multiplexing technology and the barcode of each sample is provided by the lab.

Best

 

sequencing next-gen genome • 825 views
ADD COMMENTlink modified 3.0 years ago by glihm560 • written 3.0 years ago by m.koohi.m70
2
gravatar for glihm
3.0 years ago by
glihm560
France
glihm560 wrote:

Hi, 

if you are looking for OTUs from sequencing data in order to compare microbial communities in your samples, you will only need FastQ files from sequencing. 

ADD COMMENTlink modified 3.0 years ago • written 3.0 years ago by glihm560
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