I am trying to figure out when the comparison between FPKM is performed in
NOTEST clause is invoked when both values are close. If one of the values is zero, there should be no testing for significance (or so it seems). However, using version
2.2.1, I get several examples where
-inf log fold change results in a significant q-value (always 0.047).
I have checked, and for all of such cases, I get no reads for the transcripts in question.
#regular case - no objections here OK 2.7692 0 -inf -nan 0.0011 0.226273 #weird case - not sure why this is "significant" OK 2.71508 0 -inf -nan 5e-05 0.0470179 yes
Is there a general case where 0 expression is tested?