How to make my nucleotide sequence as a BLAST database against my targeted sequence

Question

[suhalsuhaimi]

hi all,

I am currently working with consensus sequence of scaffold/matrix attachment regions (S/MARs) from Chinese hamster ovary (CHO) cells. Is it possible to make these consensus sequences as a database? If it is possible, can I do BLAST against the database as the S/MARs criteria will be the targeted sequence in order to determine whether those consensus sequences that I get matched with the criteria that I have? What kind of BLAST can I do?