error when running trimmomatic
1
1
Entering edit mode
6.1 years ago
A ★ 4.0k

hi friends,

i was going to adapter trimming by trimmomatic then i did so but ...

[izadi@lbox161 ~]$ cd /usr/data/nfs6/izadi/Trimmomatic-0.33/
[izadi@lbox161 Trimmomatic-0.33]$ java -jar trimmomatic-0.30.jar SE -phred33 SRR1944913.fastq output.fq ILLUMINACLIP:TruSeq3-SE:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36
Error: Unable to access jarfile trimmomatic-0.30.jar

do you know what happened?

thank you

 

adapter trimmomatic • 4.7k views
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2
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6.1 years ago
GenoMax 109k

Going to guess that since you are in directory for Trimmomatic-0.33 you should be using "java -jar trimmomatic-0.33.jar" instead of trimmomatic-0.30.jar?

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sorry you means I should used

trimmomatic-0.30.jar SE -phred33 SRR1944913.fastq output.fq ILLUMINACLIP:TruSeq3-SE:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36

instead of

java -jar trimmomatic-0.30.jar SE -phred33 SRR1944913.fastq output.fq ILLUMINACLIP:TruSeq3-SE:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36

??

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1
Entering edit mode

Jar file has the same number as the version of the program. You appear to be in v.0.33 directory but are trying to run v.0.30 jar.

trimmomatic-0.33.jar SE -phred33 SRR1944913.fastq output.fq ILLUMINACLIP:TruSeq3-SE:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36
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