Entering edit mode
8.5 years ago
tonja.r
▴
600
In DESeq one can normalize the read counts using estimateSizeFactor
and then look at the dispersion of the gene using estimateDispersion
. The normalized counts can be extracted with counts(cds,normalized=TRUE)
. And the dispersion graph with the plotDispEsts
.
I have other normalization methods (for instance edgeR) and I would like to check for the dispersion there or to check how the normalization of the data worked. What is the best way to show it? I do not think that plotting the distribution of the normalized counts for each sample for each normalization would be comparable somehow.
If you have your data on an ExpressionSet object, you can use boxplot() on it.