I am trying to submit bacterial genomes to NCBI.
I use 'tbl2asn' to create .sqn file using the .fsa and the .tbl files obtained from the annotation I did using PROKKA.
However, I obtained many errors concerning the locus-tag.
Here some errors reported in the discrep file as example:
FATAL: DiscRep_ALL:INCONSISTENT_LOCUS_TAG_PREFIX::4718 features have locus tag prefix AD2.
FATAL: DiscRep_ALL:DISC_SOURCE_QUALS_ASNDISC::strain (all present, some duplicate)
DiscRep_SUB:DISC_SOURCE_QUALS_ASNDISC::169 sources have 'AD2' for strain
I looked into the help NCBI provide but I cannot really understand where is the problem and how to fix it.