Question: I need a help about DNA microarray analysis please
gravatar for libya.tahani
5.1 years ago by
libya.tahani10 wrote:

Hello all,

After preprocessing & analysis of DNA microarray data , How could I benefit from this data to get a solution for some issues, for example diagnosis or detect breast cancer in the sample of the experiment  ?  


I'm new in bioinformatics field and I have a research about using DNA microarray technology for early diagnosis or detect some disease (using R & Bioconductor) . and my research is not in Lab but it is as a simulation on computer only .


genome R gene • 1.2k views
ADD COMMENTlink modified 5.1 years ago by MrT110 • written 5.1 years ago by libya.tahani10

How did you perform an analysis of the data without an end result in mind?

ADD REPLYlink written 5.1 years ago by _r_am30k
gravatar for MrT11
5.1 years ago by
MrT110 wrote:

What kind of data output did you get? Gene IDs & Fold Change? you could sort your genes starting off with setting a threshold for a FC of your interest, and the direction (up or down-regulation).

After this you can sort your list using some clustering online tool such as DAVID through which you can cluster your genes of interest per disease for instance - cancer.

After that you see which kind of molecule you are interested in and try to sort even further. For instance by trying protein-protein interaction with a pathway of your interest which is particularly overexpressed in your ideal setting.

Hope it helps. cheers

ADD COMMENTlink written 5.1 years ago by MrT110

Thanks a lot for your help ..

ADD REPLYlink written 5.1 years ago by libya.tahani10
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1676 users visited in the last hour