Shrinking fold change and dispersion for RNA-seq data ( DESeq2)

Question

[schmilblik]

Hello biostars,

I'm new to RNA-seq and I'm trying to introduce myself to DESeq2.

My question is: Why do we need to shrink the fold change and dispersion for RNA-seq data?

Thanks in advance

Answer 1

[Michael Love]

I'd recommend trying to read the DESeq2 paper from 2014 (we wrote it with non-statisticians in mind), but you might also try this post on another thread:

A: Can someone please explain in simple terms how DESeq2 works?