Please help me about the average insert size for paired end library (100bp) made by dUTP method on Illumina platform. The sequencing center told us “from the bioanalyzer results, the cDNA fragment have been cut targeting approximately 160 bp usually the standard deviation is approximately 40bp”. Could you please help me to correctly know the average insert size?
I adjusted the size of 200 bp for SOAP-de novo-trans assembler, but since too much contig remained as singletons (don’t located in the scaffold), I’m in a doubt for right average insert size. Thank you for any comments and suggestion.