Illumina Raw sequencing data conversion to single fastq file
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6.1 years ago

I was given data in two formats, one in 18 fastq files separated as each pair end, and then raw data files in .txt that look like fastq file except without the .fastq at the end. I am trying to find the best way to convert these files to fastq.gz for use with basespace.

illumina fastq raw data RNA-Seq • 2.8k views
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6.1 years ago
Brice Sarver ★ 3.7k
gzip [yourfile.fastq]

yields

[yourfile.fastq].gz
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So all I need to do is add .fastq to then end and then gzip it? Also what is the best tool to demultiplex the data?

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Entering edit mode
6.1 years ago

So all I need to do is add .fastq to then end and then gzip it? Also what is the best tool to demultiplex the data?

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